Bibliographic Details
| Title: |
Efecto de crecimiento in vitro de Nocardia spp frente a la lisozima. |
| Alternate Title: |
In vitro growth effect of Nocardia spp against lysozyme. |
| Authors: |
Palma Ramos, Alejandro1 alpalma@correo.xoc.uam.mx, Castañeda Sánchez, Jorge Ismael1, Castrillón Rivera, Laura Estela1, Arenas Guzmán, Roberto2 |
| Source: |
Dermatología Revista Mexicana. ene/feb2024, Vol. 68 Issue 1, p5-12. 8p. |
| Abstract (English): |
OBJECTIVES: To study the in vitro effect of lysozyme on six strains of Nocardia and to determine whether there is a growth inhibiting effect. MATERIALS AND METHODS: A prospective study was carried out from March to December 2022 in the Immunobiology laboratory of the Metropolitan Autonomous University, Xochimilco campus, Mexico City, in which six strains of Nocardia were grown (5 clinical isolates provided by the Manuel Gea González General Hospital and the reference strain CETC 3032) in BHI broth; they were adjusted to tube 1 of the McFarland nephelometer, and were placed in the presence of the enzyme lysozyme of human neutrophils at different concentrations and times in acetate buffer to later perform the count of microorganisms. RESULTS: Lysozyme showed a growth inhibiting effect at concentrations of 500 and 1000 µg/mL in the Nocardia brasiliensis CETC 3032 strain at 48 hours. N. brasiliensis (clinical isolate 1) and N. farcinica and N. asteroides strains (clinical isolates 3 and 4) showed inhibition of bacterial growth at all three enzyme concentrations tested at 24 hours. CONCLUSIONS: The enzyme lysozyme showed an inhibitory effect on bacterial growth on all strains of Nocardia studied. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): |
OBJETIVOS: Estudiar el efecto in vitro de la lisozima en seis cepas de Nocardia y determinar si existe efecto inhibidor del crecimiento. MATERIALES Y MÉTODOS: Estudio prospectivo efectuado de marzo a diciembre de 2022 en el laboratorio de Inmunobiología de la Universidad Autónoma Metropolitana, plantel Xochimilco, en el que se crecieron seis cepas de Nocardia (5 aislamientos clínicos proporcionados por el Hospital General Manuel Gea González y la cepa de referencia CETC 3032) en caldo BHI; se ajustaron al tubo 1 del nefelómetro de McFarland y se colocaron en presencia de la enzima lisozima de neutrófilos humanos a diferentes concentraciones y tiempos en buffer de acetatos para posteriormente realizar la cuenta de microorganismos. RESULTADOS: La lisozima mostró un efecto inhibidor del crecimiento a concentraciones de 500 y 1000 µg/mL en la cepa de Nocardia brasiliensis CETC 3032 a las 48 horas. N. brasiliensis (aislamiento clínico 1) y las cepas de N. farcinica y N. asteroides (aislamientos clínicos 3 y 4) mostraron inhibición del crecimiento bacteriano en las tres concentraciones de enzima probadas a las 24 horas. CONCLUSIONES: La enzima lisozima tuvo un efecto inhibidor del crecimiento bacteriano en todas las cepas de Nocardia estudiadas. [ABSTRACT FROM AUTHOR] |
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| Database: |
MedicLatina |
