Diagnóstico etiológico de las mastitis infecciosas: propuesta de protocolo para el cultivo de muestras de leche humana.

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Title:	Diagnóstico etiológico de las mastitis infecciosas: propuesta de protocolo para el cultivo de muestras de leche humana.
Alternate Title:	Etiological diagnosis of infectious mastitis: proposal of a protocol for the culture of human milk samples.
Authors:	Arroyo, R.¹, Mediano, P.¹, Martín, V.¹, Jiménez, E.¹, Delgado, S.¹, Fernández, L.¹, Marín, M.¹, Rodríguez, J. M.¹ jmrodrig@vet.ucm.es
Source:	Acta Pediátrica Española. jun2011, Vol. 69 Issue 6, p276-281. 6p.
Subjects:	BREAST milk, BOVINE mastitis, LACTATION, GRAM-positive bacteria, BACTERIAL diseases
Abstract (English):	During lactation, the physiological mammary microbiotas characterized by the presence of a relatively heterogeneous population, dominated by certain Gram-positive bacteria, at a moderated concentration (<1,000 CFU/mL milk). However, there are circumstances that may lead to a disbiotic process of the mammary gland microbiota, leading to mastitis. This is a widely underrated condition, partly due to the absence of uniform protocols for the collection of this biological fluid, the lack of tradition in microbiological analysis of human milk, and the doubts that sometimes arise when the results interpretation is being done. In this article, we present a basic protocol for the collection of human milk samples for their microbiological analysis and simple guidelines in order to facilitate the interpretation of the results. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	En condiciones fisiológicas, la microbiota mamaria de una mujer lactante se caracteriza por la presencia de una población relativamente heterogénea, dominada por ciertas bacterias grampositivas, en una concentración moderada (<1.000 unidades formadoras de colonias/mL de leche). Sin embargo, hay ciertas circunstancias que pueden conducir a una auténtica disbiosis de la microbiota normal de la glándula mamaria y a una mastitis. Se trata de un problema infravalorado, en gran medida por la ausencia de protocolos para la recogida de este fluido biológico, por la falta de tradición en el análisis microbiológico de la leche humana y por las dudas que suelen surgir a la hora de interpretar los resultados. En este artículo se presenta un protocolo básico para la recogida de muestras de leche humana para su análisis microbiológico y se ofrecen unas pautas sencillas para la interpretación de los resultados. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

Description
Abstract:	During lactation, the physiological mammary microbiotas characterized by the presence of a relatively heterogeneous population, dominated by certain Gram-positive bacteria, at a moderated concentration (<1,000 CFU/mL milk). However, there are circumstances that may lead to a disbiotic process of the mammary gland microbiota, leading to mastitis. This is a widely underrated condition, partly due to the absence of uniform protocols for the collection of this biological fluid, the lack of tradition in microbiological analysis of human milk, and the doubts that sometimes arise when the results interpretation is being done. In this article, we present a basic protocol for the collection of human milk samples for their microbiological analysis and simple guidelines in order to facilitate the interpretation of the results. [ABSTRACT FROM AUTHOR]
ISSN:	00016640