Mass production of active recombinant Chryseobacterium proteolyticum protein glutaminase in Escherichia coli using a sequential dual expression system and one-step purification.
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| Title: | Mass production of active recombinant Chryseobacterium proteolyticum protein glutaminase in Escherichia coli using a sequential dual expression system and one-step purification. |
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| Authors: | Lu X; Pilot Laboratory, Institute of Translational Medicine, Centre for Precision Medicine Research and Training, Faculty of Health sciences, University of Macau, Macau, China.; Department of Biology, Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research and Shenzhen Key Laboratory of Cell Microenvironment, Southern University of Science and Technology, Shenzhen, China., Poon TCW; Pilot Laboratory, Institute of Translational Medicine, Centre for Precision Medicine Research and Training, Faculty of Health sciences, University of Macau, Macau, China., Zhang H; Department of Biology, Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research and Shenzhen Key Laboratory of Cell Microenvironment, Southern University of Science and Technology, Shenzhen, China. |
| Source: | IUBMB life [IUBMB Life] 2020 Nov; Vol. 72 (11), pp. 2391-2399. Date of Electronic Publication: 2020 Aug 22. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Journal Info: | Publisher: Published for the International Union of Biochemistry and Molecular Biology by Taylor & Francis Country of Publication: England NLM ID: 100888706 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-6551 (Electronic) Linking ISSN: 15216543 NLM ISO Abbreviation: IUBMB Life Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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| ISSN: | 1521-6551 |
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| DOI: | 10.1002/iub.2358 |