Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis.
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| Title: | Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis. |
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| Authors: | Sun XM; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China., Zhang ZX; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China., Wang LR; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China., Wang JG; HuaRui Biotechnology Company, Huzhou, Zhejiang, China., Liang Y; HuaRui Biotechnology Company, Huzhou, Zhejiang, China., Yang HF; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China., Tao RS; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China., Jiang Y; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China., Yang JJ; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China., Yang S; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China. |
| Source: | Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Jan; Vol. 118 (1), pp. 153-163. Date of Electronic Publication: 2020 Sep 18. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Journal Info: | Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0290 (Electronic) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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| ISSN: | 1097-0290 |
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| DOI: | 10.1002/bit.27558 |
