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Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis.

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Title: Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis.
Authors: Sun XM; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China., Zhang ZX; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China., Wang LR; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China., Wang JG; HuaRui Biotechnology Company, Huzhou, Zhejiang, China., Liang Y; HuaRui Biotechnology Company, Huzhou, Zhejiang, China., Yang HF; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China., Tao RS; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China., Jiang Y; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China., Yang JJ; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China., Yang S; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China.
Source: Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Jan; Vol. 118 (1), pp. 153-163. Date of Electronic Publication: 2020 Sep 18.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0290 (Electronic) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis.
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  Data: <searchLink fieldCode="AU" term="%22Sun+XM%22">Sun XM</searchLink>; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China.<br /><searchLink fieldCode="AU" term="%22Zhang+ZX%22">Zhang ZX</searchLink>; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China.<br /><searchLink fieldCode="AU" term="%22Wang+LR%22">Wang LR</searchLink>; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China.<br /><searchLink fieldCode="AU" term="%22Wang+JG%22">Wang JG</searchLink>; HuaRui Biotechnology Company, Huzhou, Zhejiang, China.<br /><searchLink fieldCode="AU" term="%22Liang+Y%22">Liang Y</searchLink>; HuaRui Biotechnology Company, Huzhou, Zhejiang, China.<br /><searchLink fieldCode="AU" term="%22Yang+HF%22">Yang HF</searchLink>; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.<br /><searchLink fieldCode="AU" term="%22Tao+RS%22">Tao RS</searchLink>; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China.<br /><searchLink fieldCode="AU" term="%22Jiang+Y%22">Jiang Y</searchLink>; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China.<br /><searchLink fieldCode="AU" term="%22Yang+JJ%22">Yang JJ</searchLink>; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China.<br /><searchLink fieldCode="AU" term="%22Yang+S%22">Yang S</searchLink>; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.; Huzhou Center of Industrial Biotechnology, Shanghai Institutes for Biological Sciences, Huzhou, Zhejiang, China.
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  Data: <searchLink fieldCode="JN" term="%227502021%22">Biotechnology and bioengineering</searchLink> [Biotechnol Bioeng] 2021 Jan; Vol. 118 (1), pp. 153-163. <i>Date of Electronic Publication: </i>2020 Sep 18.
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  Data: Journal Article; Research Support, Non-U.S. Gov't
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Wiley%22">Wiley </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>7502021 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1097-0290 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200063592%22">00063592 </searchLink><i>NLM ISO Abbreviation: </i>Biotechnol Bioeng <i>Subsets: </i>MEDLINE
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        Value: 10.1002/bit.27558
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        Text: English
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              Text: 2021 Jan
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