Single-cell transcriptomics defines an improved, validated monoculture protocol for differentiation of human iPSC to microglia.
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| Title: | Single-cell transcriptomics defines an improved, validated monoculture protocol for differentiation of human iPSC to microglia. |
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| Authors: | Washer SJ; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK. sam.washer@path.ox.ac.uk.; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, UK. sam.washer@path.ox.ac.uk.; Open Targets, Wellcome Genome Campus, Hinxton, CB10 1SA, UK. sam.washer@path.ox.ac.uk., Perez-Alcantara M; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, UK.; Open Targets, Wellcome Genome Campus, Hinxton, CB10 1SA, UK., Chen Y; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, UK.; Open Targets, Wellcome Genome Campus, Hinxton, CB10 1SA, UK., Steer J; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, UK.; Open Targets, Wellcome Genome Campus, Hinxton, CB10 1SA, UK., James WS; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK., Trynka G; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, UK.; Open Targets, Wellcome Genome Campus, Hinxton, CB10 1SA, UK., Bassett AR; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, UK.; Open Targets, Wellcome Genome Campus, Hinxton, CB10 1SA, UK., Cowley SA; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK. sally.cowley@path.ox.ac.uk. |
| Source: | Scientific reports [Sci Rep] 2022 Nov 14; Vol. 12 (1), pp. 19454. Date of Electronic Publication: 2022 Nov 14. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Journal Info: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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| ISSN: | 2045-2322 |
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| DOI: | 10.1038/s41598-022-23477-2 |
