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Optimization of an infectious subgenomic amplicons reverse genetics protocol for the rescue of synthetic coronaviruses.

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Title: Optimization of an infectious subgenomic amplicons reverse genetics protocol for the rescue of synthetic coronaviruses.
Authors: Puglia I; PhD National Programme in One Health approaches to infectious diseases and life science research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Italy; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Caporale M; PhD National Programme in One Health approaches to infectious diseases and life science research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Italy; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Di Teodoro G; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Spedicato M; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Profeta F; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Marcacci M; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Di Pancrazio C; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Valleriani F; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Rossi E; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Auerswald H; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Lorusso A; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy. Electronic address: a.lorusso@izs.it.
Source: Journal of virological methods [J Virol Methods] 2025 Jul; Vol. 336, pp. 115152. Date of Electronic Publication: 2025 Apr 04.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1879-0984
DOI:10.1016/j.jviromet.2025.115152