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Optimization of an infectious subgenomic amplicons reverse genetics protocol for the rescue of synthetic coronaviruses.

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Title: Optimization of an infectious subgenomic amplicons reverse genetics protocol for the rescue of synthetic coronaviruses.
Authors: Puglia I; PhD National Programme in One Health approaches to infectious diseases and life science research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Italy; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Caporale M; PhD National Programme in One Health approaches to infectious diseases and life science research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Italy; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Di Teodoro G; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Spedicato M; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Profeta F; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Marcacci M; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Di Pancrazio C; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Valleriani F; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Rossi E; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Auerswald H; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy., Lorusso A; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy. Electronic address: a.lorusso@izs.it.
Source: Journal of virological methods [J Virol Methods] 2025 Jul; Vol. 336, pp. 115152. Date of Electronic Publication: 2025 Apr 04.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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PubType: Academic Journal
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  Data: Optimization of an infectious subgenomic amplicons reverse genetics protocol for the rescue of synthetic coronaviruses.
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  Data: <searchLink fieldCode="AU" term="%22Puglia+I%22">Puglia I</searchLink>; PhD National Programme in One Health approaches to infectious diseases and life science research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Italy; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Caporale+M%22">Caporale M</searchLink>; PhD National Programme in One Health approaches to infectious diseases and life science research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Italy; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Di+Teodoro+G%22">Di Teodoro G</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Spedicato+M%22">Spedicato M</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Profeta+F%22">Profeta F</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Marcacci+M%22">Marcacci M</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Di+Pancrazio+C%22">Di Pancrazio C</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Valleriani+F%22">Valleriani F</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Rossi+E%22">Rossi E</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Auerswald+H%22">Auerswald H</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy.<br /><searchLink fieldCode="AU" term="%22Lorusso+A%22">Lorusso A</searchLink>; Istituto Zooprofilattico Sperimentale dell' Abruzzo e Molise, Teramo, Italy. Electronic address: a.lorusso@izs.it.
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  Data: <searchLink fieldCode="JN" term="%228005839%22">Journal of virological methods</searchLink> [J Virol Methods] 2025 Jul; Vol. 336, pp. 115152. <i>Date of Electronic Publication: </i>2025 Apr 04.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier%2FNorth-Holland+Biomedical+Press%22">Elsevier/North-Holland Biomedical Press </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>8005839 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1879-0984 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2201660934%22">01660934 </searchLink><i>NLM ISO Abbreviation: </i>J Virol Methods <i>Subsets: </i>MEDLINE
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        Value: 10.1016/j.jviromet.2025.115152
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              Text: 2025 Jul
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