Development and validation of the Azu-Eos as a fluorescence-free staining method for enhanced bright-field microscopy in the comet assay.

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Title: Development and validation of the Azu-Eos as a fluorescence-free staining method for enhanced bright-field microscopy in the comet assay.
Authors: Ivanov P; Laboratory of Molecular Genetics, Epigenetics and Longevity, Institute of Molecular Biology 'Roumen Tsanev', Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria. Electronic address: p.ves.iv@gmail.com., Staneva D; Laboratory of Molecular Genetics, Epigenetics and Longevity, Institute of Molecular Biology 'Roumen Tsanev', Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria. Electronic address: dessysta@gmail.com., Georgieva M; Laboratory of Molecular Genetics, Epigenetics and Longevity, Institute of Molecular Biology 'Roumen Tsanev', Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria. Electronic address: milenageorgy@gmail.com., Miloshev G; Laboratory of Molecular Genetics, Epigenetics and Longevity, Institute of Molecular Biology 'Roumen Tsanev', Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria. Electronic address: karamolbiol@gmail.com.
Source: Methods (San Diego, Calif.) [Methods] 2025 Sep; Vol. 241, pp. 103-113. Date of Electronic Publication: 2025 May 27.
Publication Type: Journal Article; Validation Study
Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1095-9130
DOI:10.1016/j.ymeth.2025.05.011