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Designing Novel Aspartic Protease (NAP) Using Ancestral Sequence Reconstruction (ASR) for Cheesemaking.

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Title: Designing Novel Aspartic Protease (NAP) Using Ancestral Sequence Reconstruction (ASR) for Cheesemaking.
Authors: Saleem SS; Enzyme Technology and X-Ray Crystallography Laboratory, Institute of Bioscience, Universiti Putra Malaysia, VacBio 5, 43400, Serdang, Selangor, Malaysia.; Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.; Department of Medical Laboratory Science, College of Health Science, University of Duhok, Duhok, Iraq., Akinola OM; Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.; Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Rahman MBA; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Leow ATC; Enzyme Technology and X-Ray Crystallography Laboratory, Institute of Bioscience, Universiti Putra Malaysia, VacBio 5, 43400, Serdang, Selangor, Malaysia.; Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.; Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.; Biotechnology and Biobased Industry Research Program, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Noor NDM; Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.; Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.; Biotechnology and Biobased Industry Research Program, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Salleh AB; Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.; Biotechnology and Biobased Industry Research Program, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Oslan SN; Enzyme Technology and X-Ray Crystallography Laboratory, Institute of Bioscience, Universiti Putra Malaysia, VacBio 5, 43400, Serdang, Selangor, Malaysia. snurbayaoslan@upm.edu.my.; Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. snurbayaoslan@upm.edu.my.; Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. snurbayaoslan@upm.edu.my.; Biotechnology and Biobased Industry Research Program, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. snurbayaoslan@upm.edu.my.
Source: Molecular biotechnology [Mol Biotechnol] 2026 May; Vol. 68 (5), pp. 2230-2252. Date of Electronic Publication: 2025 Jul 07.
Publication Type: Journal Article
Journal Info: Publisher: Springer Country of Publication: Switzerland NLM ID: 9423533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0305 (Electronic) Linking ISSN: 10736085 NLM ISO Abbreviation: Mol Biotechnol Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1559-0305
DOI:10.1007/s12033-025-01470-0