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Protocol to isolate glycoproteins from cultured mammalian cells and tissue samples using wheat germ agglutinin precipitation.

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Title: Protocol to isolate glycoproteins from cultured mammalian cells and tissue samples using wheat germ agglutinin precipitation.
Authors: Chopra S; Department of Molecular and Experimental Nutritional Medicine, Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. Electronic address: chopra1@uni-potsdam.de., Kadiri OL; Department of Molecular and Experimental Nutritional Medicine, Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. Electronic address: otsuware.linda-josephine.kadiri@uni-potsdam.de., Kappert K; Institute of Diagnostic Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité- Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Cardiovascular-Metabolic-Renal (CMR) Research Center, Charité - Universitätsmedizin Berlin, Hessische Straße 3-4, 10115 Berlin, Germany., Kleinridders A; Department of Molecular and Experimental Nutritional Medicine, Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. Electronic address: kleinridders@uni-potsdam.de.
Source: STAR protocols [STAR Protoc] 2025 Sep 19; Vol. 6 (3), pp. 103954. Date of Electronic Publication: 2025 Jul 17.
Publication Type: Journal Article
Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:2666-1667
DOI:10.1016/j.xpro.2025.103954