Directed evolution of LaccID for cell surface proximity labeling and electron microscopy.

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Title: Directed evolution of LaccID for cell surface proximity labeling and electron microscopy.
Authors: Lee SY; Department of Genetics, Stanford University, Stanford, CA, USA.; Department of New Biology, DGIST, Daegu, Republic of Korea.; New Biology Research Center, DGIST, Daegu, Republic of Korea., Roh H; Department of Chemistry, Stanford University, Stanford, CA, USA.; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA., Gonzalez-Perez D; Department of Biocatalysis, Institute of Catalysis, ICP-CSIC, Madrid, Spain., Mackey MR; Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA.; Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA, USA., Hoces D; Department of Genetics, Stanford University, Stanford, CA, USA.; Department of Bioengineering, Stanford University, Stanford, CA, USA., McLaughlin CN; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA., Lin C; Department of Genetics, Stanford University, Stanford, CA, USA., Adams SR; Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA., Nguyen K; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Kim KY; Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA., Luginbuhl DJ; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA., Luo L; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA., Udeshi ND; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Carr SA; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Hernández-López RA; Department of Genetics, Stanford University, Stanford, CA, USA.; Department of Bioengineering, Stanford University, Stanford, CA, USA.; Chan Zuckerberg Biohub - San Francisco, San Francisco, CA, USA., Ellisman MH; Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA.; Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA, USA., Alcalde M; Department of Biocatalysis, Institute of Catalysis, ICP-CSIC, Madrid, Spain., Ting AY; Department of Genetics, Stanford University, Stanford, CA, USA. ayting@stanford.edu.; Department of Chemistry, Stanford University, Stanford, CA, USA. ayting@stanford.edu.; Chan Zuckerberg Biohub - San Francisco, San Francisco, CA, USA. ayting@stanford.edu.; Department of Biology, Stanford University, Stanford, CA, USA. ayting@stanford.edu.; The Phil & Penny Knight Initiative for Brain Resilience at the Wu Tsai Neurosciences Institute, Stanford, CA, USA. ayting@stanford.edu.
Source: Nature chemical biology [Nat Chem Biol] 2025 Dec; Vol. 21 (12), pp. 1895-1905. Date of Electronic Publication: 2025 Aug 01.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101231976 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4469 (Electronic) Linking ISSN: 15524450 NLM ISO Abbreviation: Nat Chem Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1552-4469
DOI:10.1038/s41589-025-01973-6