Analytical dissection of minor glycoforms and glycoprotein associations in rAAV preparations by multimodal glycoproteomics.

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Title: Analytical dissection of minor glycoforms and glycoprotein associations in rAAV preparations by multimodal glycoproteomics.
Authors: Kuno A; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan. atsu-kuno@aist.go.jp., Sakaue H; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan., Koizumi S; Precision System Science Co. Ltd., 88 Kamihongo, Matsudo, Chiba, 271-0064, Japan., Tomioka A; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan., Mizukado S; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan., Yamaguchi Y; Department of Biotechnology, Graduate School of Engineering, The University of Osaka, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan., Fukuhara M; Department of Biotechnology, Graduate School of Engineering, The University of Osaka, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan., Tsunaka Y; Department of Biotechnology, Graduate School of Engineering, The University of Osaka, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan., Kaji H; Institute for Glyco-Core Research (iGCORE), Nagoya University, Furo-Cho, Chikusa, Nagoya, Aichi, 464-8601, Japan., Uchiyama S; Department of Biotechnology, Graduate School of Engineering, The University of Osaka, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. suchi@bio.eng.osaka-u.ac.jp.
Source: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2025 Sep; Vol. 417 (23), pp. 5155-5170. Date of Electronic Publication: 2025 Aug 07.
Publication Type: Journal Article
Journal Info: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1618-2650
DOI:10.1007/s00216-025-06042-4