Protocol for pooled FACS-based CRISPR knockout screening in human iPSC-derived microglia.

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Title: Protocol for pooled FACS-based CRISPR knockout screening in human iPSC-derived microglia.
Authors: Washer SJ; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK; Open Targets, Wellcome Genome Campus, Hinxton CB10 1SA, UK; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK. Electronic address: sam.washer@cmd.ox.ac.uk., Navarro-Guerrero E; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK., Cowley SA; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK., Ebner DV; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK., Bassett AR; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK; Open Targets, Wellcome Genome Campus, Hinxton CB10 1SA, UK.
Source: STAR protocols [STAR Protoc] 2025 Dec 19; Vol. 6 (4), pp. 104111. Date of Electronic Publication: 2025 Sep 20.
Publication Type: Journal Article
Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:2666-1667
DOI:10.1016/j.xpro.2025.104111