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Protocol for pooled FACS-based CRISPR knockout screening in human iPSC-derived microglia.

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Title: Protocol for pooled FACS-based CRISPR knockout screening in human iPSC-derived microglia.
Authors: Washer SJ; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK; Open Targets, Wellcome Genome Campus, Hinxton CB10 1SA, UK; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK. Electronic address: sam.washer@cmd.ox.ac.uk., Navarro-Guerrero E; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK., Cowley SA; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK., Ebner DV; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK., Bassett AR; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK; Open Targets, Wellcome Genome Campus, Hinxton CB10 1SA, UK.
Source: STAR protocols [STAR Protoc] 2025 Dec 19; Vol. 6 (4), pp. 104111. Date of Electronic Publication: 2025 Sep 20.
Publication Type: Journal Article
Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Washer+SJ%22">Washer SJ</searchLink>; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK; Open Targets, Wellcome Genome Campus, Hinxton CB10 1SA, UK; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK. Electronic address: sam.washer@cmd.ox.ac.uk.<br /><searchLink fieldCode="AU" term="%22Navarro-Guerrero+E%22">Navarro-Guerrero E</searchLink>; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK.<br /><searchLink fieldCode="AU" term="%22Cowley+SA%22">Cowley SA</searchLink>; James and Lillian Martin Centre for Stem Cell Research, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.<br /><searchLink fieldCode="AU" term="%22Ebner+DV%22">Ebner DV</searchLink>; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Old Road Campus, Oxford OX3 7FZ, UK.<br /><searchLink fieldCode="AU" term="%22Bassett+AR%22">Bassett AR</searchLink>; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton CB10 1SA, UK; Open Targets, Wellcome Genome Campus, Hinxton CB10 1SA, UK.
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  Data: <searchLink fieldCode="JN" term="%22101769501%22">STAR protocols</searchLink> [STAR Protoc] 2025 Dec 19; Vol. 6 (4), pp. 104111. <i>Date of Electronic Publication: </i>2025 Sep 20.
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        Value: 10.1016/j.xpro.2025.104111
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              Text: 2025 Dec 19
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