Establishment and application of six-plex fluorescent quantitative PCR assay for the detection of calf diarrhea pathogens.

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Title: Establishment and application of six-plex fluorescent quantitative PCR assay for the detection of calf diarrhea pathogens.
Authors: Niu Z; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China., Nie L; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China., Yan J; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China., Xu X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China., Hou X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; JUNLEBAO-Northwest A&F University Cooperation Dairy Research Institute, Leyuan Animal Husbandry, JUNLEBAO Company, Shijiazhuang 050200, China., Huang Y; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China., Du Q; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China. Electronic address: dewey3600@nwsuaf.edu.cn., Tong D; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China. Electronic address: dwtong@nwsuaf.edu.cn.
Source: Journal of virological methods [J Virol Methods] 2026 Apr; Vol. 341, pp. 115323. Date of Electronic Publication: 2025 Dec 08.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1879-0984
DOI:10.1016/j.jviromet.2025.115323