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Establishment and application of six-plex fluorescent quantitative PCR assay for the detection of calf diarrhea pathogens.

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Title: Establishment and application of six-plex fluorescent quantitative PCR assay for the detection of calf diarrhea pathogens.
Authors: Niu Z; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China., Nie L; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China., Yan J; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China., Xu X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China., Hou X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; JUNLEBAO-Northwest A&F University Cooperation Dairy Research Institute, Leyuan Animal Husbandry, JUNLEBAO Company, Shijiazhuang 050200, China., Huang Y; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China., Du Q; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China. Electronic address: dewey3600@nwsuaf.edu.cn., Tong D; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China. Electronic address: dwtong@nwsuaf.edu.cn.
Source: Journal of virological methods [J Virol Methods] 2026 Apr; Vol. 341, pp. 115323. Date of Electronic Publication: 2025 Dec 08.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Establishment and application of six-plex fluorescent quantitative PCR assay for the detection of calf diarrhea pathogens.
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  Data: <searchLink fieldCode="AU" term="%22Niu+Z%22">Niu Z</searchLink>; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.<br /><searchLink fieldCode="AU" term="%22Nie+L%22">Nie L</searchLink>; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.<br /><searchLink fieldCode="AU" term="%22Yan+J%22">Yan J</searchLink>; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.<br /><searchLink fieldCode="AU" term="%22Xu+X%22">Xu X</searchLink>; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.<br /><searchLink fieldCode="AU" term="%22Hou+X%22">Hou X</searchLink>; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; JUNLEBAO-Northwest A&F University Cooperation Dairy Research Institute, Leyuan Animal Husbandry, JUNLEBAO Company, Shijiazhuang 050200, China.<br /><searchLink fieldCode="AU" term="%22Huang+Y%22">Huang Y</searchLink>; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China.<br /><searchLink fieldCode="AU" term="%22Du+Q%22">Du Q</searchLink>; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China. Electronic address: dewey3600@nwsuaf.edu.cn.<br /><searchLink fieldCode="AU" term="%22Tong+D%22">Tong D</searchLink>; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education, Yangling 712100, China; Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agriculture and Rural Affairs, Yangling 712100, China; Engineering Research Center of Efficient New Vaccines for Animals, Universities of Shaanxi Province, Yangling 712100, China. Electronic address: dwtong@nwsuaf.edu.cn.
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  Data: <searchLink fieldCode="JN" term="%228005839%22">Journal of virological methods</searchLink> [J Virol Methods] 2026 Apr; Vol. 341, pp. 115323. <i>Date of Electronic Publication: </i>2025 Dec 08.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier%2FNorth-Holland+Biomedical+Press%22">Elsevier/North-Holland Biomedical Press </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>8005839 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1879-0984 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2201660934%22">01660934 </searchLink><i>NLM ISO Abbreviation: </i>J Virol Methods <i>Subsets: </i>MEDLINE
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.jviromet.2025.115323
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      – Code: eng
        Text: English
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        StartPage: 115323
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      – TitleFull: Establishment and application of six-plex fluorescent quantitative PCR assay for the detection of calf diarrhea pathogens.
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          Name:
            NameFull: Niu Z
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            NameFull: Nie L
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            NameFull: Yan J
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            NameFull: Xu X
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            NameFull: Hou X
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            NameFull: Huang Y
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            NameFull: Du Q
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            NameFull: Tong D
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          Dates:
            – D: 01
              M: 04
              Text: 2026 Apr
              Type: published
              Y: 2026
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              Value: 1879-0984
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              Value: 341
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            – TitleFull: Journal of virological methods
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