Artifact-free preparation of biological samples for SEM by optimized water freeze-drying.

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Title: Artifact-free preparation of biological samples for SEM by optimized water freeze-drying.
Authors: Ishida H; Graduate School of Natural Science and Technology, Shimane University, 1060 Nishikawatsu-cho, Matsue, 690-8504, Japan. h-ishida@life.shimane-u.ac.jp., Fukuda N; Graduate School of Natural Science and Technology, Shimane University, 1060 Nishikawatsu-cho, Matsue, 690-8504, Japan., Shimada M; Graduate School of Natural Science and Technology, Shimane University, 1060 Nishikawatsu-cho, Matsue, 690-8504, Japan., Yamamoto K; Graduate School of Natural Science and Technology, Shimane University, 1060 Nishikawatsu-cho, Matsue, 690-8504, Japan., Gaponova L; Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan.; Laboratory of Preservation and Renewal of Biodiversity, Institute for Evolutionary Ecology of the National Academy of Sciences of Ukraine, Kyiv, 03143, Ukraine., Higuchi R; Department of Biology, Faculty of Science, Konan University, 8-9-1, Okamoto, Higashinada, Kobe, 658-8501, Japan., Kolosiuk A; Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan.; Laboratory of Radiation Technology, Institute of Physics of the National Academy of Sciences of Ukraine, Kyiv, 03028, Ukraine., Hoshina R; Nagahama Institute of Bio-Science and Technology, Tamura 1266, Nagahama, 526-0829, Shiga, Japan., Arikawa M; Department of Biological Sciences, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono, Kochi, 780-8520, Japan., Fukuda Y; Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Narukoonsen, Osaki, 989-6711, Miyagi, Japan., Islam MDS; Ansar/VDP Training Center, Dhaka, Bangladesh., Harumoto T; Nara Women's University, Kita-Uoya-Nishi-machi, Nara, 630-8506, Japan., Wan Y; Xiangya School of Basic Medical Sciences, Central South University, No. 172 Tongzipo Road, Yuelu District, Changsha City, Hunan Province, China.; Division of Membrane Biology, Department of Biochemistry and Molecular Biology, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan., Itoh T; Biosignal Research Center, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan.; Division of Membrane Biology, Department of Biochemistry and Molecular Biology, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan., Inai Y; Department of Biochemistry, Wakayama Medical University, 811-1 Kimiidera, Wakayama, 641-0012, Japan., Takeishi A; Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan., Aonuma H; Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan., Kikuchi T; Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan., Nishigori S; Department of Materials Analysis, Interdisciplinary Center for Science Research, Shimane University, 1060 Nishikawatsu-cho, Matsue, 690-8504, Japan., Maruyama T; School of Marine Biosciences, Kitasato University, Sagamihara, 252-0373, Kanagawa, Japan., Ikeda K; Graduate School of Agrobiological Science, Kobe University, 1-1 Rokkodai- cho, Nada-ku, Kobe, 657-8501, Japan., Iriko H; Division of Global Infectious Diseases, Department of Public Health, Graduate School of Health Sciences, Kobe University, 7-10-2 Tomogaoka, Suma-ku, Kobe, 654-0142, Japan., Suzaki T; Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan.
Source: Scientific reports [Sci Rep] 2025 Dec 10; Vol. 16 (1), pp. 717. Date of Electronic Publication: 2025 Dec 10.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:2045-2322
DOI:10.1038/s41598-025-30335-4