HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects.

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Title: HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects.
Authors: Shirai Y; Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.; Department of Organismic & Evolutionary Biology, Harvard University, Cambridge, MA, USA., Kao JA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA., Kumar T; Department of Organismic & Evolutionary Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Matsuda N; Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan., Nakagawa R; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan., Nureki O; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan., Extavour CG; Department of Organismic & Evolutionary Biology, Harvard University, Cambridge, MA, USA.; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Daimon T; Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. daimon.takaaki.7a@kyoto-u.ac.jp.
Source: Communications biology [Commun Biol] 2026 Mar 03; Vol. 9 (1). Date of Electronic Publication: 2026 Mar 03.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:2399-3642
DOI:10.1038/s42003-026-09777-7