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Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.

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Title: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
Authors: Tseng TY; Department of Chemical Engineering, National Cheng Kung University, No. 1, University Road, Tainan City, 70101, Taiwan., Mai HJ; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan City, 32003, Taiwan., Yan ST; Taiwan RedEye Biomedical Inc., Hsinchu City, 300, Taiwan., Tsai HF; Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, Tainan City, 70146, Taiwan., Chen Y; Graduate Institute of Medicine, Yuan Ze University, Taoyuan City, 32003, Taiwan.; Department of Surgery, Far Eastern Memorial Hospital, New Taipei City, 22060, Taiwan., Yao CL; Department of Chemical Engineering, National Cheng Kung University, No. 1, University Road, Tainan City, 70101, Taiwan. yao8@gs.ncku.edu.tw.; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan City, 32003, Taiwan. yao8@gs.ncku.edu.tw.
Source: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2026 Apr; Vol. 49 (4), pp. 985-997. Date of Electronic Publication: 2026 Mar 04.
Publication Type: Journal Article
Journal Info: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-7605 (Electronic) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1615-7605
DOI:10.1007/s00449-026-03300-2