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Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.

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Title: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
Authors: Tseng TY; Department of Chemical Engineering, National Cheng Kung University, No. 1, University Road, Tainan City, 70101, Taiwan., Mai HJ; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan City, 32003, Taiwan., Yan ST; Taiwan RedEye Biomedical Inc., Hsinchu City, 300, Taiwan., Tsai HF; Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, Tainan City, 70146, Taiwan., Chen Y; Graduate Institute of Medicine, Yuan Ze University, Taoyuan City, 32003, Taiwan.; Department of Surgery, Far Eastern Memorial Hospital, New Taipei City, 22060, Taiwan., Yao CL; Department of Chemical Engineering, National Cheng Kung University, No. 1, University Road, Tainan City, 70101, Taiwan. yao8@gs.ncku.edu.tw.; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan City, 32003, Taiwan. yao8@gs.ncku.edu.tw.
Source: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2026 Apr; Vol. 49 (4), pp. 985-997. Date of Electronic Publication: 2026 Mar 04.
Publication Type: Journal Article
Journal Info: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-7605 (Electronic) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
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  Data: <searchLink fieldCode="AU" term="%22Tseng+TY%22">Tseng TY</searchLink>; Department of Chemical Engineering, National Cheng Kung University, No. 1, University Road, Tainan City, 70101, Taiwan.<br /><searchLink fieldCode="AU" term="%22Mai+HJ%22">Mai HJ</searchLink>; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan City, 32003, Taiwan.<br /><searchLink fieldCode="AU" term="%22Yan+ST%22">Yan ST</searchLink>; Taiwan RedEye Biomedical Inc., Hsinchu City, 300, Taiwan.<br /><searchLink fieldCode="AU" term="%22Tsai+HF%22">Tsai HF</searchLink>; Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, Tainan City, 70146, Taiwan.<br /><searchLink fieldCode="AU" term="%22Chen+Y%22">Chen Y</searchLink>; Graduate Institute of Medicine, Yuan Ze University, Taoyuan City, 32003, Taiwan.; Department of Surgery, Far Eastern Memorial Hospital, New Taipei City, 22060, Taiwan.<br /><searchLink fieldCode="AU" term="%22Yao+CL%22">Yao CL</searchLink>; Department of Chemical Engineering, National Cheng Kung University, No. 1, University Road, Tainan City, 70101, Taiwan. yao8@gs.ncku.edu.tw.; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan City, 32003, Taiwan. yao8@gs.ncku.edu.tw.
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  Data: <searchLink fieldCode="JN" term="%22101088505%22">Bioprocess and biosystems engineering</searchLink> [Bioprocess Biosyst Eng] 2026 Apr; Vol. 49 (4), pp. 985-997. <i>Date of Electronic Publication: </i>2026 Mar 04.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Springer-Verlag%22">Springer-Verlag </searchLink><i>Country of Publication: </i>Germany <i>NLM ID: </i>101088505 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1615-7605 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2216157591%22">16157591 </searchLink><i>NLM ISO Abbreviation: </i>Bioprocess Biosyst Eng <i>Subsets: </i>MEDLINE
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      – Type: doi
        Value: 10.1007/s00449-026-03300-2
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      – Code: eng
        Text: English
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      – TitleFull: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
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            NameFull: Tseng TY
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            NameFull: Mai HJ
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            NameFull: Yan ST
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            NameFull: Tsai HF
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              Text: 2026 Apr
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              Y: 2026
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              Value: 49
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            – TitleFull: Bioprocess and biosystems engineering
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