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A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce.

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Title: A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce.
Authors: Di Pinto A; National Research Council (CNR), Institute of Molecular Biology and Pathology (IBPM), 00185 Rome, Italy.; Department of Molecular Medicine, University La Sapienza, 00161 Rome, Italy., Forte V; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy., D'Attilia C; National Research Council (CNR), Institute of Agricultural Biology and Biotechnology (IBBA), 00010 Rome, Italy.; Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, 01100 Viterbo, Italy., Possenti M; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy., Felici B; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy., Augelletti F; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy., Sessa G; National Research Council (CNR), Institute of Molecular Biology and Pathology (IBPM), 00185 Rome, Italy., Carabelli M; National Research Council (CNR), Institute of Molecular Biology and Pathology (IBPM), 00185 Rome, Italy., Morelli G; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy.; Accademia Nazionale dei Lincei, 00165 Rome, Italy., Frugis G; National Research Council (CNR), Institute of Agricultural Biology and Biotechnology (IBBA), 00010 Rome, Italy., D'Orso F; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy.
Source: Plants (Basel, Switzerland) [Plants (Basel)] 2026 Apr 09; Vol. 15 (8). Date of Electronic Publication: 2026 Apr 09.
Publication Type: Journal Article
Journal Info: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101596181 Publication Model: Electronic Cited Medium: Print ISSN: 2223-7747 (Print) Linking ISSN: 22237747 NLM ISO Abbreviation: Plants (Basel) Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Di+Pinto+A%22">Di Pinto A</searchLink>; National Research Council (CNR), Institute of Molecular Biology and Pathology (IBPM), 00185 Rome, Italy.; Department of Molecular Medicine, University La Sapienza, 00161 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Forte+V%22">Forte V</searchLink>; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22D'Attilia+C%22">D'Attilia C</searchLink>; National Research Council (CNR), Institute of Agricultural Biology and Biotechnology (IBBA), 00010 Rome, Italy.; Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, 01100 Viterbo, Italy.<br /><searchLink fieldCode="AU" term="%22Possenti+M%22">Possenti M</searchLink>; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Felici+B%22">Felici B</searchLink>; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Augelletti+F%22">Augelletti F</searchLink>; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Sessa+G%22">Sessa G</searchLink>; National Research Council (CNR), Institute of Molecular Biology and Pathology (IBPM), 00185 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Carabelli+M%22">Carabelli M</searchLink>; National Research Council (CNR), Institute of Molecular Biology and Pathology (IBPM), 00185 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Morelli+G%22">Morelli G</searchLink>; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy.; Accademia Nazionale dei Lincei, 00165 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22Frugis+G%22">Frugis G</searchLink>; National Research Council (CNR), Institute of Agricultural Biology and Biotechnology (IBBA), 00010 Rome, Italy.<br /><searchLink fieldCode="AU" term="%22D'Orso+F%22">D'Orso F</searchLink>; Council for Agricultural Research and Economics (CREA), Research Centre for Genomics and Bioinformatics, 00178 Rome, Italy.
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  Data: <searchLink fieldCode="JN" term="%22101596181%22">Plants (Basel, Switzerland)</searchLink> [Plants (Basel)] 2026 Apr 09; Vol. 15 (8). <i>Date of Electronic Publication: </i>2026 Apr 09.
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              Text: 2026 Apr 09
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