Development and field evaluation of a rapid detection method for Babesia microti using fluorescent recombinase polymerase amplification(RPA) technology.

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Title: Development and field evaluation of a rapid detection method for Babesia microti using fluorescent recombinase polymerase amplification(RPA) technology.
Authors: Cai Y; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China., Yang HY; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China., Qiang K; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China., Yu C; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China., Yan S; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China., Mo Z; The Institutes of Biomedical Sciences, College of Life Sciences, Inner Mongolia University, Hohhot, 010000, China., Zheng B; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China. zhengbin@nipd.chinacdc.cn.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China. zhengbin@nipd.chinacdc.cn.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China. zhengbin@nipd.chinacdc.cn.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China. zhengbin@nipd.chinacdc.cn., Xu B; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China. xubin@nipd.chinacdc.cn.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China. xubin@nipd.chinacdc.cn.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China. xubin@nipd.chinacdc.cn.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China. xubin@nipd.chinacdc.cn., Hu W; The Institutes of Biomedical Sciences, College of Life Sciences, Inner Mongolia University, Hohhot, 010000, China. huw@fudan.edu.cn.; School of Life Sciences, Fudan University, Shanghai, 200433, China. huw@fudan.edu.cn.
Source: Scientific reports [Sci Rep] 2026 May 05; Vol. 16 (1). Date of Electronic Publication: 2026 May 05.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
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  Data: Development and field evaluation of a rapid detection method for Babesia microti using fluorescent recombinase polymerase amplification(RPA) technology.
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  Data: <searchLink fieldCode="AU" term="%22Cai+Y%22">Cai Y</searchLink>; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China.<br /><searchLink fieldCode="AU" term="%22Yang+HY%22">Yang HY</searchLink>; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China.<br /><searchLink fieldCode="AU" term="%22Qiang+K%22">Qiang K</searchLink>; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China.<br /><searchLink fieldCode="AU" term="%22Yu+C%22">Yu C</searchLink>; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China.<br /><searchLink fieldCode="AU" term="%22Yan+S%22">Yan S</searchLink>; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China.<br /><searchLink fieldCode="AU" term="%22Mo+Z%22">Mo Z</searchLink>; The Institutes of Biomedical Sciences, College of Life Sciences, Inner Mongolia University, Hohhot, 010000, China.<br /><searchLink fieldCode="AU" term="%22Zheng+B%22">Zheng B</searchLink>; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China. zhengbin@nipd.chinacdc.cn.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China. zhengbin@nipd.chinacdc.cn.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China. zhengbin@nipd.chinacdc.cn.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China. zhengbin@nipd.chinacdc.cn.<br /><searchLink fieldCode="AU" term="%22Xu+B%22">Xu B</searchLink>; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China. xubin@nipd.chinacdc.cn.; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai, 200025, China. xubin@nipd.chinacdc.cn.; Laboratory of Parasite and Vector Biology, Ministry of Public Health, Shanghai, 200025, China. xubin@nipd.chinacdc.cn.; WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China. xubin@nipd.chinacdc.cn.<br /><searchLink fieldCode="AU" term="%22Hu+W%22">Hu W</searchLink>; The Institutes of Biomedical Sciences, College of Life Sciences, Inner Mongolia University, Hohhot, 010000, China. huw@fudan.edu.cn.; School of Life Sciences, Fudan University, Shanghai, 200433, China. huw@fudan.edu.cn.
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        Value: 10.1038/s41598-026-42288-3
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      – TitleFull: Development and field evaluation of a rapid detection method for Babesia microti using fluorescent recombinase polymerase amplification(RPA) technology.
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              Text: 2026 May 05
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