Use of the Golden Gate Cloning Technique for Plasmid Construction to Generate Conditional Lethal Mutants in C. difficile.
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| Title: | Use of the Golden Gate Cloning Technique for Plasmid Construction to Generate Conditional Lethal Mutants in C. difficile. |
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| Authors: | Malet-Villemagne J; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France., Zielinski ME; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France.; Hôpital de la Pitié Salpêtrière, Laboratoire de virologie, Paris, France., Parfut A; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France.; Plateau technique de Microbiologie, CHU de Strasbourg, Strasbourg, France., Candela T; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France. thomas.candela@inrae.fr. |
| Source: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 3046, pp. 29-45. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
| ISSN: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-5328-9_3 |
