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Use of the Golden Gate Cloning Technique for Plasmid Construction to Generate Conditional Lethal Mutants in C. difficile.

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Title: Use of the Golden Gate Cloning Technique for Plasmid Construction to Generate Conditional Lethal Mutants in C. difficile.
Authors: Malet-Villemagne J; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France., Zielinski ME; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France.; Hôpital de la Pitié Salpêtrière, Laboratoire de virologie, Paris, France., Parfut A; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France.; Plateau technique de Microbiologie, CHU de Strasbourg, Strasbourg, France., Candela T; Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, France. thomas.candela@inrae.fr.
Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 3046, pp. 29-45.
Publication Type: Journal Article
Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Use of the Golden Gate Cloning Technique for Plasmid Construction to Generate Conditional Lethal Mutants in C. difficile.
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