Inhibitory Effect of Spirodela polyrhixa on the Secretion of NO in LPS-stimulated Macrophages.

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Bibliographic Details
Title:	Inhibitory Effect of Spirodela polyrhixa on the Secretion of NO in LPS-stimulated Macrophages.
Authors:	Woo Shin Ko, Young Hee Kim, Jung Won Yoon, So Woon Yoon, Han Do Kim
Source:	American Journal of Chinese Medicine. 2004, Vol. 32 Issue 1, p65-73. 9p. 1 Diagram, 2 Graphs.
Subjects:	Spirodela, Duckweeds, Spirodela polyrrhiza, Nitrogen compounds, Amic acids
Abstract:	Spirodela polyrhixa Schleid has been used in folk medicine to treat inflammatory diseases. Since nitric oxide (NO) is one of the major inflammatory parameters, we studied the effect of aqueous extracts of Spirodela polyrhixa (AESP) on NO production in lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages. AESP inhibited the secretion of NO in macrophages, without affecting cell viability. The protein level of inducible nitric oxide synthase (iNOS) in peritoneal macrophages was also decreased by AESP. Transient transfection assay of reporter plasmid and gel shift assay indicated that AESP blocked the activation of nuclear factor-kappa B (NF-κB), which was considered to be a potential transcription factor for iNOS expression. AESP also blocked the phosphorylation and degradation of inhibitory protein I kappa B-alpha (IκB-α). These results suggest that AESP could exert its anti-inflammatory actions by suppressing the synthesis of NO through inhibition of NF-κB activity. [ABSTRACT FROM AUTHOR]
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Database:	Psychology and Behavioral Sciences Collection

Description
Abstract:	Spirodela polyrhixa Schleid has been used in folk medicine to treat inflammatory diseases. Since nitric oxide (NO) is one of the major inflammatory parameters, we studied the effect of aqueous extracts of Spirodela polyrhixa (AESP) on NO production in lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages. AESP inhibited the secretion of NO in macrophages, without affecting cell viability. The protein level of inducible nitric oxide synthase (iNOS) in peritoneal macrophages was also decreased by AESP. Transient transfection assay of reporter plasmid and gel shift assay indicated that AESP blocked the activation of nuclear factor-kappa B (NF-κB), which was considered to be a potential transcription factor for iNOS expression. AESP also blocked the phosphorylation and degradation of inhibitory protein I kappa B-alpha (IκB-α). These results suggest that AESP could exert its anti-inflammatory actions by suppressing the synthesis of NO through inhibition of NF-κB activity. [ABSTRACT FROM AUTHOR]
ISSN:	0192415X
DOI:	10.1142/S0192415X04001795