Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology.
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| Title: | Bacterial pathogen deploys the iminosugar glycosyrin to manipulate plant glycobiology. |
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| Authors: | Sanguankiattichai, Nattapong, Chandrasekar, Balakumaran, Sheng, Yuewen, Hardenbrook, Nathan, Tabak, Werner W. A., Drapal, Margit, Kaschani, Farnusch, Grünwald-Gruber, Clemens, Krahn, Daniel, Buscaill, Pierre, Yamamoto, Suzuka, Kato, Atsushi, Nash, Robert, Fleet, George, Strasser, Richard, Fraser, Paul D., Kaiser, Markus, Zhang, Peijun, Preston, Gail M., van der Hoorn, Renier A. L. |
| Source: | Science. 4/18/2025, Vol. 388 Issue 6744, p297-303. 7p. |
| Subjects: | Bacterial diseases, Iminosugars, Glycomics, Phytopathogenic microorganisms, Flagellin, Glycosylation |
| Abstract: | The extracellular space (apoplast) in plants is a key battleground during microbial infections. To avoid recognition, the bacterial model phytopathogen Pseudomonas syringae pv. tomato DC3000 produces glycosyrin. Glycosyrin inhibits the plant-secreted β-galactosidase BGAL1, which would otherwise initiate the release of immunogenic peptides from bacterial flagellin. Here, we report the structure, biosynthesis, and multifunctional roles of glycosyrin. High-resolution cryo–electron microscopy and chemical synthesis revealed that glycosyrin is an iminosugar with a five-membered pyrrolidine ring and a hydrated aldehyde that mimics monosaccharides. Glycosyrin biosynthesis was controlled by virulence regulators, and its production is common in bacteria and prevents flagellin recognition and alters the extracellular glycoproteome and metabolome of infected plants. These findings highlight a potentially wider role for glycobiology manipulation by plant pathogens across the plant kingdom. Editor's summary: Plants and their pathogens continually undergo a molecular arms race. Sanguankiattichai et al. found that the bacterial plant pathogen Pseudomonas syringae inhibits a plant-secreted glycosidase that would otherwise initiate the release of flagellin fragments that are recognized by the plant to trigger its defenses (see the Perspective by Schroeder). The authors performed high-resolution cryo–electron microscopy and chemical synthesis studies to show that this glycosidase inhibitor, glycosyrin, is a distinct iminosugar that mimics galactose. Glycosyrin biosynthesis was induced by virulence regulators during the infection process and produced by a distinct iminosugar biosynthesis pathway. Glycosyrin also altered the glycosylation of plant proteins and the accumulation of extracellular metabolites. Many plant-associated bacteria can produce glycosyrin, which suggests that glycobiology manipulation may represent a common battleground during plant-pathogen interactions. —Stella M. Hurtley [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
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| Abstract: | The extracellular space (apoplast) in plants is a key battleground during microbial infections. To avoid recognition, the bacterial model phytopathogen Pseudomonas syringae pv. tomato DC3000 produces glycosyrin. Glycosyrin inhibits the plant-secreted β-galactosidase BGAL1, which would otherwise initiate the release of immunogenic peptides from bacterial flagellin. Here, we report the structure, biosynthesis, and multifunctional roles of glycosyrin. High-resolution cryo–electron microscopy and chemical synthesis revealed that glycosyrin is an iminosugar with a five-membered pyrrolidine ring and a hydrated aldehyde that mimics monosaccharides. Glycosyrin biosynthesis was controlled by virulence regulators, and its production is common in bacteria and prevents flagellin recognition and alters the extracellular glycoproteome and metabolome of infected plants. These findings highlight a potentially wider role for glycobiology manipulation by plant pathogens across the plant kingdom. Editor's summary: Plants and their pathogens continually undergo a molecular arms race. Sanguankiattichai et al. found that the bacterial plant pathogen Pseudomonas syringae inhibits a plant-secreted glycosidase that would otherwise initiate the release of flagellin fragments that are recognized by the plant to trigger its defenses (see the Perspective by Schroeder). The authors performed high-resolution cryo–electron microscopy and chemical synthesis studies to show that this glycosidase inhibitor, glycosyrin, is a distinct iminosugar that mimics galactose. Glycosyrin biosynthesis was induced by virulence regulators during the infection process and produced by a distinct iminosugar biosynthesis pathway. Glycosyrin also altered the glycosylation of plant proteins and the accumulation of extracellular metabolites. Many plant-associated bacteria can produce glycosyrin, which suggests that glycobiology manipulation may represent a common battleground during plant-pathogen interactions. —Stella M. Hurtley [ABSTRACT FROM AUTHOR] |
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| ISSN: | 00368075 |
| DOI: | 10.1126/science.adp2433 |
