SALICYLIC ACID SENSOR1 reveals the propagation of an SA hormone surge during plant pathogen advance.
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| Title: | SALICYLIC ACID SENSOR1 reveals the propagation of an SA hormone surge during plant pathogen advance. |
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| Authors: | Tang, Bijun, Lu, Jing, Leontovyčová, Hana, Hoffmann, Gesa, Rowe, James H., O'Donnell, Sacha Fouquay, Grangé-Guermente, Mathieu, Larsen, Bo, Wimalasekera, Rinukshi, Carella, Philip, Incarbone, Marco, Kalachova, Tetiana, Jones, Alexander M. |
| Source: | Science. 10/9/2025, Vol. 390 Issue 6769, p188-194. 7p. |
| Subjects: | Salicylic acid, Plant hormones, Phytopathogenic microorganisms, Fluorescence resonance energy transfer, Biosensors, Bacterial diseases |
| Abstract: | Salicylic acid (SA) is a key phytohormone that orchestrates immune responses against pathogens, including Pseudomonas syringae bacteria. The timing and extent of SA accumulation are tightly controlled by plants but can be suppressed by pathogens to overcome immunity. Understanding SA dynamics at high spatiotemporal resolution remains challenging owing to limitations in existing detection methods that are indirect, destructive, or lacking in cellular precision and temporal resolution. We developed SalicS1, a genetically encoded fluorescence resonance energy transfer (FRET) biosensor specific to SA. SalicS1 enables real-time, reversible monitoring of SA levels in vivo with minimal perturbation of endogenous signaling. We reveal the propagation of an SA surge spreading from bacterial infection sites with spatiotemporal fidelity. SalicS1 unlocks precise understanding of SA dynamics underpinning crop resilience to pathogens. Editor's summary: Visualizing and quantifying the amount of a hormone within a cell can be achieved using fluorescence resonance energy transfer (FRET) sensors. These sensors involve a hormone binding to a pair of linked proteins, which then undergo conformational changes to alter the fluorescence emission ratio of two connected fluorescent proteins. Tang et al. have developed a FRET sensor for the plant hormone salicylic acid, which is primarily involved in activating immune responses. Using this sensor, the authors were able to visualize hormone levels with high sensitivity and spatial resolution at the cellular level and observed varied dynamics and patterns of salicylic acid up-regulation in response to bacterial infections. —Madeleine Seale [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
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| Abstract: | Salicylic acid (SA) is a key phytohormone that orchestrates immune responses against pathogens, including Pseudomonas syringae bacteria. The timing and extent of SA accumulation are tightly controlled by plants but can be suppressed by pathogens to overcome immunity. Understanding SA dynamics at high spatiotemporal resolution remains challenging owing to limitations in existing detection methods that are indirect, destructive, or lacking in cellular precision and temporal resolution. We developed SalicS1, a genetically encoded fluorescence resonance energy transfer (FRET) biosensor specific to SA. SalicS1 enables real-time, reversible monitoring of SA levels in vivo with minimal perturbation of endogenous signaling. We reveal the propagation of an SA surge spreading from bacterial infection sites with spatiotemporal fidelity. SalicS1 unlocks precise understanding of SA dynamics underpinning crop resilience to pathogens. Editor's summary: Visualizing and quantifying the amount of a hormone within a cell can be achieved using fluorescence resonance energy transfer (FRET) sensors. These sensors involve a hormone binding to a pair of linked proteins, which then undergo conformational changes to alter the fluorescence emission ratio of two connected fluorescent proteins. Tang et al. have developed a FRET sensor for the plant hormone salicylic acid, which is primarily involved in activating immune responses. Using this sensor, the authors were able to visualize hormone levels with high sensitivity and spatial resolution at the cellular level and observed varied dynamics and patterns of salicylic acid up-regulation in response to bacterial infections. —Madeleine Seale [ABSTRACT FROM AUTHOR] |
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| ISSN: | 00368075 |
| DOI: | 10.1126/science.adw7650 |
