View in EDS PDF Full Text

Gravitational unloading inhibits the regenerative potential of atrophied soleus muscle in mice.

Saved in:
Bibliographic Details
Title: Gravitational unloading inhibits the regenerative potential of atrophied soleus muscle in mice.
Authors: Matsuba, Y., Goto, K., Morioka, S., Naito, T., Akema, T., Hashimoto, N., Sugiura, T., Ohira, Y., Beppu, M., Yoshioka, T.
Source: Acta Physiologica. Jul2009, Vol. 196 Issue 3, p329-339. 11p. 2 Diagrams, 5 Graphs.
Subjects: Regenerative medicine, Regeneration (Biology), Muscle contraction, Muscle diseases, Compartment syndrome, Contracture (Pathology), Rat diseases
Abstract: Aim: The present study was performed to investigate the influence of unloading on the regeneration of atrophied and injured skeletal muscle. Methods: Male mice (C57BL/6J), aged 8 weeks, were used. Cardiotoxin (CTX) was injected into soleus muscles bilaterally. Gravitational unloading on soleus muscle was performed by hind limb suspension for 2 weeks before and additionally 6 weeks after CTX injection in one group. Soleus muscles in the remaining groups were loaded keeping the mice in the cages and were dissected 14, 28 and 42 days after the injection. Results: Recovery of the wet weight and protein content of soleus in the CTX-injected group was inhibited by unloading. Increase in satellite cell number, induced by CTX injection and loading, was also inhibited by unloading. Disappearance of infiltration of mononucleated cells into the necrotic area was also delayed. This phenomenon suggests that regeneration, which is indicated by the appearance of fibres with central nuclei, was inhibited by unloading. Conclusion: Results suggested that loading plays an important role in the activation of the regenerating potential of injured skeletal muscle. [ABSTRACT FROM AUTHOR]
Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
Description
Abstract:Aim: The present study was performed to investigate the influence of unloading on the regeneration of atrophied and injured skeletal muscle. Methods: Male mice (C57BL/6J), aged 8 weeks, were used. Cardiotoxin (CTX) was injected into soleus muscles bilaterally. Gravitational unloading on soleus muscle was performed by hind limb suspension for 2 weeks before and additionally 6 weeks after CTX injection in one group. Soleus muscles in the remaining groups were loaded keeping the mice in the cages and were dissected 14, 28 and 42 days after the injection. Results: Recovery of the wet weight and protein content of soleus in the CTX-injected group was inhibited by unloading. Increase in satellite cell number, induced by CTX injection and loading, was also inhibited by unloading. Disappearance of infiltration of mononucleated cells into the necrotic area was also delayed. This phenomenon suggests that regeneration, which is indicated by the appearance of fibres with central nuclei, was inhibited by unloading. Conclusion: Results suggested that loading plays an important role in the activation of the regenerating potential of injured skeletal muscle. [ABSTRACT FROM AUTHOR]
ISSN:17481708
DOI:10.1111/j.1748-1716.2008.01943.x