Molecular characterization and gene expression of Na -K -2Cl cotransporter2 (NKCC2) in the gastrointestinal tract of Olive flounder ( Paralichythys olivaceus ) during the four days after infection with Streptococcus parauberis.
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| Title: | Molecular characterization and gene expression of Na -K -2Cl cotransporter2 (NKCC2) in the gastrointestinal tract of Olive flounder ( Paralichythys olivaceus ) during the four days after infection with Streptococcus parauberis. |
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| Authors: | Kim, YiKyung (AUTHOR), Watanabe, Soichi (AUTHOR), Park, Sooil (AUTHOR), Jeong, JoonBum (AUTHOR), Kaneko, Toyoji (AUTHOR), Park, Myeong Ae (AUTHOR), Yeo, In Kyu (AUTHOR) |
| Source: | Marine & Freshwater Behaviour & Physiology. May2013, Vol. 46 Issue 3, p145-157. 13p. 2 Diagrams, 1 Chart, 3 Graphs. |
| Subjects: | Gene expression, Molecular genetics, Ion channels, Gastrointestinal diseases, Paralichthyidae, Streptococcus, Bacterial diseases |
| Abstract: | This study reports on the molecular characterization and gene expression of Na+-K+-2Cl−cotransporter2 (NKCC2) in the gastrointestinal tract of Olive flounderParalichythys olivaceusinfected withStreptococcus parauberis. It examines the electroneutral cation-Cl−cotransporter, and NKCC2 expressed in the intestinal tract. First, we isolated cDNA encoding NKCC2 from the intestinal tract of Olive flounder and investigated the tissue distribution pattern of NKCC2 mRNA. Reverse transcription-PCR analysis detected expressions of NKCC2 in the intestine and kidney. A quantitative PCR analysis showed that the expression level of NKCC2 in the anterior and posterior intestines was higher than in the rectum. Second, we performed a challenge experiment comparing physiological saline (PS) and PS plus the bacteria and analyzed the subsequent gene expression of NKCC2. At 4 days after bacterial injection the comparison between treatment and control found that the expression levels of NKCC2 mRNA increased significantly in the posterior intestine of infected fish but not in the other segments. We discuss the suggestion that bacterial injection may disturb the ion uptake that provides the driving force for water absorption through parts of the intestinal wall. [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
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| Abstract: | This study reports on the molecular characterization and gene expression of Na+-K+-2Cl−cotransporter2 (NKCC2) in the gastrointestinal tract of Olive flounderParalichythys olivaceusinfected withStreptococcus parauberis. It examines the electroneutral cation-Cl−cotransporter, and NKCC2 expressed in the intestinal tract. First, we isolated cDNA encoding NKCC2 from the intestinal tract of Olive flounder and investigated the tissue distribution pattern of NKCC2 mRNA. Reverse transcription-PCR analysis detected expressions of NKCC2 in the intestine and kidney. A quantitative PCR analysis showed that the expression level of NKCC2 in the anterior and posterior intestines was higher than in the rectum. Second, we performed a challenge experiment comparing physiological saline (PS) and PS plus the bacteria and analyzed the subsequent gene expression of NKCC2. At 4 days after bacterial injection the comparison between treatment and control found that the expression levels of NKCC2 mRNA increased significantly in the posterior intestine of infected fish but not in the other segments. We discuss the suggestion that bacterial injection may disturb the ion uptake that provides the driving force for water absorption through parts of the intestinal wall. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 10236244 |
| DOI: | 10.1080/10236244.2013.793472 |
