Differential effects of antipsychotics on the development of rat oligodendrocyte precursor cells exposed to cuprizone.
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| Title: | Differential effects of antipsychotics on the development of rat oligodendrocyte precursor cells exposed to cuprizone. |
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| Authors: | Xu, Haiyun, Yang, Hong-Ju, Li, Xin-Min |
| Source: | European Archives of Psychiatry & Clinical Neuroscience. Mar2014, Vol. 264 Issue 2, p121-129. 9p. |
| Subjects: | Brain damage, Antipsychotic agents, White matter (Nerve tissue), Oligodendroglia, Copper chelates, Laboratory rats, Drug efficacy, Progenitor cells |
| Abstract: | Cuprizone (CPZ) is a copper-chelating agent and has been shown to induce white matter damage in mice and rats. The compromised white matter and oligodendrocytes (OLs) respond to some antipsychotics in vivo. However, little is known about the effects of antipsychotics on cultured OLs in the presence of CPZ. The aim of this study was to examine effects of some antipsychotics on developing OLs in the presence of CPZ. Oligodendrocyte progenitor cells (OPCs) were prepared from rat embryos; OLs at different developing stages were labeled with specific antibodies; levels of CNP and MBP proteins in mature OLs were assessed by Western-blot analysis; malondialdehyde (MDA) levels and activity of catalase were evaluated as well for an assessment of oxidative stress and antioxidative status. In immunofluorescent staining, CPZ was shown to inhibit the differentiation of cultured OPCs into O4-positive cells, reduce the maturation of O4-positive cells into CNP- and MBP-positive cells, and decrease levels of CNP and MBP in mature OLs. These inhibitory effects of CPZ were ameliorated by clozapine and quetiapine (QUE), but not by haloperidol and olanzapine. Further experiments were performed to explore the mechanism of the protective effects of QUE. QUE attenuated the decreases in CNP and MBP in CPZ-treated OLs, and blocked the CPZ-induced increase in MDA and decrease in catalase activity in cultured OLs. These results are relevant to the pathophysiology and treatment of schizophrenia considering the aberrant white matter development and evidence suggesting the derangement of the oxidant and antioxidant defense system in some of the patients with schizophrenia. [ABSTRACT FROM AUTHOR] |
| Copyright of European Archives of Psychiatry & Clinical Neuroscience is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 94448963 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Differential effects of antipsychotics on the development of rat oligodendrocyte precursor cells exposed to cuprizone. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Xu%2C+Haiyun%22">Xu, Haiyun</searchLink><br /><searchLink fieldCode="AR" term="%22Yang%2C+Hong-Ju%22">Yang, Hong-Ju</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Xin-Min%22">Li, Xin-Min</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Archives+of+Psychiatry+%26+Clinical+Neuroscience%22">European Archives of Psychiatry & Clinical Neuroscience</searchLink>. Mar2014, Vol. 264 Issue 2, p121-129. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Brain+damage%22">Brain damage</searchLink><br /><searchLink fieldCode="DE" term="%22Antipsychotic+agents%22">Antipsychotic agents</searchLink><br /><searchLink fieldCode="DE" term="%22White+matter+%28Nerve+tissue%29%22">White matter (Nerve tissue)</searchLink><br /><searchLink fieldCode="DE" term="%22Oligodendroglia%22">Oligodendroglia</searchLink><br /><searchLink fieldCode="DE" term="%22Copper+chelates%22">Copper chelates</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+rats%22">Laboratory rats</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+efficacy%22">Drug efficacy</searchLink><br /><searchLink fieldCode="DE" term="%22Progenitor+cells%22">Progenitor cells</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Cuprizone (CPZ) is a copper-chelating agent and has been shown to induce white matter damage in mice and rats. The compromised white matter and oligodendrocytes (OLs) respond to some antipsychotics in vivo. However, little is known about the effects of antipsychotics on cultured OLs in the presence of CPZ. The aim of this study was to examine effects of some antipsychotics on developing OLs in the presence of CPZ. Oligodendrocyte progenitor cells (OPCs) were prepared from rat embryos; OLs at different developing stages were labeled with specific antibodies; levels of CNP and MBP proteins in mature OLs were assessed by Western-blot analysis; malondialdehyde (MDA) levels and activity of catalase were evaluated as well for an assessment of oxidative stress and antioxidative status. In immunofluorescent staining, CPZ was shown to inhibit the differentiation of cultured OPCs into O4-positive cells, reduce the maturation of O4-positive cells into CNP- and MBP-positive cells, and decrease levels of CNP and MBP in mature OLs. These inhibitory effects of CPZ were ameliorated by clozapine and quetiapine (QUE), but not by haloperidol and olanzapine. Further experiments were performed to explore the mechanism of the protective effects of QUE. QUE attenuated the decreases in CNP and MBP in CPZ-treated OLs, and blocked the CPZ-induced increase in MDA and decrease in catalase activity in cultured OLs. These results are relevant to the pathophysiology and treatment of schizophrenia considering the aberrant white matter development and evidence suggesting the derangement of the oxidant and antioxidant defense system in some of the patients with schizophrenia. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Archives of Psychiatry & Clinical Neuroscience is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00406-013-0414-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 121 Subjects: – SubjectFull: Brain damage Type: general – SubjectFull: Antipsychotic agents Type: general – SubjectFull: White matter (Nerve tissue) Type: general – SubjectFull: Oligodendroglia Type: general – SubjectFull: Copper chelates Type: general – SubjectFull: Laboratory rats Type: general – SubjectFull: Drug efficacy Type: general – SubjectFull: Progenitor cells Type: general Titles: – TitleFull: Differential effects of antipsychotics on the development of rat oligodendrocyte precursor cells exposed to cuprizone. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Xu, Haiyun – PersonEntity: Name: NameFull: Yang, Hong-Ju – PersonEntity: Name: NameFull: Li, Xin-Min IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2014 Type: published Y: 2014 Identifiers: – Type: issn-print Value: 09401334 Numbering: – Type: volume Value: 264 – Type: issue Value: 2 Titles: – TitleFull: European Archives of Psychiatry & Clinical Neuroscience Type: main |
| ResultId | 1 |
